| Dersin İçeriği | Çevresel Kirlenme, Moleküler Biyoloji ve Genotoksisiteye Giriş, Bakterillerin Genel Özellikleri-Suş Mekanizması, In-vivo ve In Vitro Yöntemler, Çevresel Ortamlardaki Genotoksisite Çalışmaları, SOS Chromotest Hücresel Olaylar ve İndüksiyon Faktör, SOS Chromotest Deney Methodolojisi, Genotoksik Aktivitenin (β-galaktosidaz, Alkalin fosfatez, Indüksiyon faktör) Hesaplanması, İstatistiksel Yaklaşım, Non-Lineer Modelleme Yaklaşımı, Ames Test, Mutatox, Umu Test, Vitotox. |
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| Ders Kitabı / Malzemesi / Önerilen Kaynaklar | - Turner, P.C., McLennan, A.G., Bates, A.D. ve White, M.R.H. (2004), Moleculer Biology, Instant Notes, University of Liverpool, UK.
- Koçak E., (2010), Haliç’teki Toplam Kirliliğin Canlı DNA’sı Üzerindeki Genotoksik Etkilerinin İncelenmesi, YTÜ FBE Doktora Tezi, İstanbul, Türkiye.
- Kocak E., Yetilmezsoy K., Gonullu M.T., Petek M., (2010), A statistical evaluation of the potential genotoxic activity in the surface waters of the Golden Horn Estuary, Marine Pollution Bulletin, 60(2010), 1708-1717.
- Quillardet, P., Huisman, O., D’Ari, R., Hofnung, M., 1982. SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K12 to measure genotoxicity. Proc. Natl. Acad. Sci. 79, 5971–5975.
- Cachot, J., Geffard, O., Augagneur, S., Lacroix, S., Le Menach, K., Peluhet, L., Couteau, J., Denier, X., Devier, M.H., Pottier, D., Budzinski, H., 2006. Evidence of genotoxicity related to high PAH content of sediments in the upper part of the Seine Estuary (Normandy, France). Aquat. Toxicol. 79, 257–267.
- Environmental Bio. Detection Products Inc. (EBPI), The SOS-Chromotest Version 6.3, Instruction for Use, Canada, 2008.
- Fish, F., Lampert, I., Halachmi, A., Riesenfeld, G., Herzberg, M., 1987. The SOS chromotest kit: a rapid method for the detection of genotoxicity. Toxic. Assess. 2, 135–147.
- Guzzella, L., Monarc, S., Zani, C., Feretti, D., Zerbini, I., Buschini, A., Poli, P., Rossi, C., Richardson, S.D., 2004. In vitro potential genotoxic effects of surface drinking water treated with chlorine and alternative disinfectants. Mutat. Res. 564, 179– 193.
- Isidori, M., Lavorgna, M., Nardelli, A., Parrella, A., 2004. Integrated environmental assessment of Volturno River in South Italy. Sci. Total Environ. 327, 123–134.
- Isidori, M., Nardelli, A., Parrella, A., Pascarella, L., Previtera, L., 2006. Multispecies study to assess the toxic and genotoxic effect of pharmaceuticals: furosemide and its photoproduct. Chemosphere 63, 785–793.
- Legault, R., Blake, C., Trottier, S., 1996. Detecting genotoxic activity in industrial effluents using the SOS chromotest microplate assay. Environ. Toxic. Water Quality 11, 151–165.
- Quillardet, P., Hofnung, M., 1985. The SOS chromotest, a colorimetric bacterial assay for genotoxins: procedures. Mutat. Res. 147, 65–78.
- Zani, C., Feretti, D., Buschini, A., Poli, P., Rossi, C., Guzzella, L., Ceternio, D.F., Monarca, S., 2005. Toxicity and genotoxicity of surface water before and after various potabilization steps. Mutat. Res. 587, 26–37.
- Ruiz, M.J., Marzin, D., 1997. Genotoxicity of six pesticides by Salmonella mutagenicity test and SOS chromotest. Mutat. Res. 390, 245–255.
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